班别 |
生技041-042 |
人数 |
38 |
实验教师 |
周玲艳 |
实验总时数 |
24 |
实验指导书 |
校内教材 |
编著者 |
周玲艳 等 |
周别/星期 |
第节 |
实验
序号 |
实验项目名称 |
实验
时数 |
每组
人数 |
主要使 用仪器 |
药品实验材料
消耗定额（每组） |
价格
元/组 |
| 3/三 |
10-12 |
1 |
Meiosis |
3 |
1 |
compound microscope; microscope slides; cover glasses; watch glass; dissecting needles; scalpel or razor blade; forceps; alcohol lamp; |
Material: male spike of maize
Reagents: acetocarmine or aceto-orcein stain in dropping bottle; Carnoy’s fixation solution; |
6.00 |
| 4/三 |
10-12 |
2 |
Karyotype Analysis of Plant |
3 |
1 |
microscope with photographic apparatus; cover glasses; watch glass; beakers; scissors; ruler. |
Material: onion bulbs
Reagents: acetocarmine stain
1M HCl, colchicine solution. |
6.00 |
| 5/三 |
10-12 |
3 |
Lucubation of Drosophila |
3 |
1 |
bottles of culture medium; gummed labels; beaker; electric stove. |
Material: The stock of wild-type fly; the stocks of mutant-type flies
Reagents: propionic acid; agar; cornmeal; sucrose; yeast; distilled water. |
6.50 |
| 6/三 |
10-12 |
4 |
The Observation of Life Cycle and Morphological Characters of Drosophila |
3 |
1 |
stereo dissecting microscope; etherizer; teasing needle or fine camel's hair brush |
Material: The stock of wild-type fly; the stocks of mutant-type flies
Reagents: ether |
5.00 |
| 7/三 |
10-12 |
5 |
Dihybrid Crosses of Drosophila |
3 |
1 |
bottles of medium for the culture of Drosophila; gummed labels; etherizer; re-etherizer; dropper; teasing needle or fine camel’s hair brush; the morgue with 70% ethyl alcohol. |
Material: The stock of wild-type fly; the stocks of mutant-type flies
Reagents: ether; 70% ethyl alcohol. |
4.50 |
| 8/三 |
?10-12 |
6 |
Structural Aberration of Chromosome |
3 |
1 |
compound microscope; cover glasses; watch glass; dissecting needles; scalpel or razor blade; forceps; alcohol lamp. |
Material: onion bulbs treated with γ radiation of Co60.
Reagents: acetocarmine stain in dropping bottle; 1N HCl; Carnoy’s fixation solution. |
7.50 |
| 9/三 |
?10-12 |
7 |
Extraction of plant DNA |
3 |
1 |
beaker; glass stirring rod; 50ml plastic screwcap centrifuge tubes; centrifuge; vortex agitator; refrigerator; mortar. |
Material: leaves of rice, corn, cabbage and so on
Reagents: 3M sodium acetate; freezer-cold 95% ethanol; EDTA; SDS; chloroform; isoamyl alcohol; Na citrate; NaCl. |
15.00 |
| 10/三 |
10-12 |
8 |
Genetic Analysis of
Quantitative Characters |
3 |
1 |
calculating device; meter rule. |
Material:rice. |
5.00 |
